Pancreatic Regenerating Gene Overexpression in the Nonobese Diabetic Mouse During Active Diabetogenesis

Author:

Baeza Nathalie J1,Moriscot Christine I1,Renaud Wanda P1,Okamoto Hiroshi2,Figarella Catherine G1,Vialettes Bernard H3

Affiliation:

1. Research Group on Exocrine Glands, Faculty of Medicine Marseille, France

2. Department of Biochemistry, Tohoku University School of Medicine Sendai, Japan

3. Laboratory of Diabetology, Timone Hospital Marseille, France

Abstract

The reg gene has previously been shown to be associated with regeneration of pancreatic islets. Strategies for influencing the replication and the growth of the β-cell mass may be important for prevention and/or treatment of type I diabetes. In this study, we have examined the level of reg gene expression at various degrees of diabetogenesis in the pancreas of the NOD mouse (male, female, and cyclophosphamide-treated male) using both human reg cDNA as the probe and dot blot analysis. The expression of the reg gene was found to be significantly increased in female mice compared with male mice, and in both cases, the expression level was not influenced by age. Nondiabetic female mice have a significantly higher expression of the gene than diabetic female mice, and there was a positive correlation between the age of diabetes onset and the reg mRNA level. In addition, overexpression of the reg gene was found in male mice treated by cyclophosphamide, an agent known to be a potent inducer of diabetes in male NOD mice. None of these results were found in the diabetes-resistant control OF1 mice, in which pancreatic reg gene expression did not differ between female and male mice treated or untreated with cyclophosphamide. All of these data suggest that there is a strong correlation between reg gene expression in the pancreas of the NOD mouse and the likelihood of developing diabetes.

Publisher

American Diabetes Association

Subject

Endocrinology, Diabetes and Metabolism,Internal Medicine

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