Culturable Bacterial Communities Related to Different Larval Stages of Sanys irrosea (Guenee, 1852) (Lepidoptera: Noctuoidae)

Abstract

Many bacterial species are frequently associated with insects in symbiotic, mutualistic, or parasitic relationships. Symbiotic bacteria living in mostly insect gut have many roles in insect’s biology such as nutrition, development, sex determination and evolution. Therefore, studying of symbiotic bacteria in insects (especially beneficial and harmful species) is very important to elucidate their roles in their hosts biology. In this study, we purposed to isolate and identify the culturable bacterial species in internal organs (mostly gut parts) of Sanys irrosea (Guenee, 1852) (Lepidoptera: Noctuoidae). The bacterial flora of different development stages of Sanys irrosea (Guenee, 1852) (Lepidoptera: Noctuoidae) was studied by culture dependent techniques and the isolated bacteria was identified by 16S rRNA sequencing and phylogenetic analysis. A total of 22 bacterial isolates were obtained from different instar larvae of the insect and were identified based on the 16S rRNA sequence analysis. Consequently, many bacterial species were identified, and Staphylococcus, Micrococcus and Bacillus species were dominant. In addi-tion, some potential slug, human and plant pathogenic bacteria (Moraxella osloensis, Kocuria rosea and Clavi-bacter michiganensis) were isolated. The results were discussed with respect to the bacterial composition of S. irrosea regarding effects of bacterial diversity on larval development of the insect and transmission of some im-portant pathogens via insect-based vectors. Results obtained from this study should be beneficial for future studies to understand roles of bacteria in larval development of Lepidopteran insects