The Heat-Shock Element Is a Functional Component of the Arabidopsis APX1 Gene Promoter

Author:

Storozhenko Sergei1,De Pauw Pascal1,Van Montagu Marc1,Inzé Dirk2,Kushnir Sergei1

Affiliation:

1. Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie (S.S., P.D.P., M.V.M., S.K.)

2. Laboratoire Associé de l'Institut National de la Recherche Agronomique (France) (D.I.), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

Abstract

Abstract Ascorbate peroxidases are important enzymes that detoxify hydrogen peroxide within the cytosol and chloroplasts of plant cells. To better understand their role in oxidative stress tolerance, the transcriptional regulation of theapx1 gene from Arabidopsis was studied. The apx1 gene was expressed in all tested organs of Arabidopsis; mRNA levels were low in roots, leaves, and stems and high in flowers. Steady-state mRNA levels in leaves or cell suspensions increased after treatment with methyl viologen, ethephon, high temperature, and illumination of etiolated seedlings. A putative heat-shock cis element found in the apx1promoter was shown to be recognized by the tomato (Lycopersicon esculentum) heat-shock factor in vitro and to be responsible for the in vivo heat-shock induction of the gene. The heat-shockcis element also contributed partially to the induction of the gene by oxidative stress. By using in vivo dimethyl sulfate footprinting, we showed that proteins interacted with a G/C-rich element found in the apx1 promoter.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

Reference59 articles.

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3. The cauliflower mosaic virus 35S promoter: combinatorial regulation of transcription in plants.;Benfey;Science,1990

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