Auxin Biosynthesis in Maize Kernels

Author:

Glawischnig Erich1,Tomas Adriana2,Eisenreich Wolfgang3,Spiteller Peter4,Bacher Adelbert3,Gierl Alfons1

Affiliation:

1. Lehrstuhl für Genetik (E.G., A.G.) and

2. Pioneer Hi-Bred International, 7250 NW 62nd Avenue, Johnston, Iowa 50131–0552 (A.T.); and

3. Lehrstuhl für Organische Chemie und Biochemie (W.E., A.B.), Technische Universität München, Lichtenbergstrasse 4, 85747 Garching, Germany;

4. Institut für Organische Chemie, Ludwig-Maximilian-Universität München, Butenandstrasse 13, 81377 München, Germany (P.S.)

Abstract

Abstract Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention of the 3,3′ bond. Conversion of Trp to IAA is not competed by indole. Labeling with the general precursors [U-13C6]glucose and [1,2-13C2]acetate followed by retrobiosynthetic analysis strongly suggest that Trp-dependent IAA synthesis is the predominant route for auxin biosynthesis in the maize kernel. The synthesis of IAA from indole glycerol phosphate and IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite can be excluded.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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4. Auxin biosynthesis.;Bartel;Annu Rev Plant Physiol Plant Mol Biol,1997

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