Purification and Characterization of Two Voltage-Dependent Anion Channel Isoforms from Plant Seeds-Reference-Cited by-同舟云学术

Purification and Characterization of Two Voltage-Dependent Anion Channel Isoforms from Plant Seeds

Published:2000-11-01 Issue:3 Volume:124 Page:1181-1190
ISSN:1532-2548
Container-title:Plant Physiology
language:en
Short-container-title:

Author:

Abrecht Helge¹,Wattiez Ruddy²,Ruysschaert Jean-Marie³,Homblé Fabrice¹

Affiliation:

1. Laboratoire de Physiologie Végétale (H.A., F.H.) and

2. Laboratoire de Biochimie, Faculté des Sciences, Université de Mons-Hainaut, B–7000 Mons, Belgium (R.W.)

3. Laboratoire de Chimie Physique des Macromolécules aux Interfaces (J.-M.R.), Faculté des Sciences, UniversitéLibre de Bruxelles, B–1050 Brussels, Belgium; and

Abstract

Abstract Mitochondria were isolated from imbibed seeds of lentil (Lens culinaris) and Phaseolus vulgaris. We copurified two voltage-dependent anion channel from detergent solubilized mitochondria in a single purification step using hydroxyapatite. The two isoforms from P. vulgaris were separated by chromatofocusing chromatography in 4m urea without any loss of channel activity. Channel activity of each isoform was characterized upon reconstitution into diphytanoyl phosphatidylcholine planar lipid bilayers. Both isoforms form large conductance channels that are slightly anion selective and display cation selective substates.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

Link

http://academic.oup.com/plphys/article-pdf/124/3/1181/38675971/plphys_v124_3_1181.pdf

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