Affiliation:
1. Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norin Kenkyu Danchinai, Ibaraki 305, Japan
Abstract
Abstract
Acetylated trigalacturonides and rhamnogalacturonan I (RG-I)-derived oligosaccharides were isolated from a Driselase digest of potato tuber cell walls by ion-exchange and size-exclusion chromatography. The oligosaccharides were structurally characterized by fast atom bombardment-mass spectroscopy, nuclear magnetic resonance spectroscopy, and glycosyl-linkage composition analysis. One trigalacturonide contained a single acetyl group at O–3 of the reducing galacturonic acid residue. A second trigalacturonide contained two acetyl substituents, which were located on O–3 or O–4 of the nonreducing galacturonic acid residue and O–3 of the reducing galacturonic acid residue. RG-I backbone-derived oligomers had acetyl groups at O–2 of the galacturonic acid residues. Some of these galacturonic acid residues were O-acetylated at both O–2 and O–3 positions. Rhamnosyl residues of RG-I oligomers were not acetylated.
Publisher
Oxford University Press (OUP)
Subject
Plant Science,Genetics,Physiology
Cited by
119 articles.
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