Assessment of Mechanical Properties of Adherent Living Cells by Bead Micromanipulation: Comparison of Magnetic Twisting Cytometry vs Optical Tweezers-Reference-Cited by-同舟云学术

Assessment of Mechanical Properties of Adherent Living Cells by Bead Micromanipulation: Comparison of Magnetic Twisting Cytometry vs Optical Tweezers

Published:2002-07-30 Issue:4 Volume:124 Page:408-421
ISSN:0148-0731
Container-title:Journal of Biomechanical Engineering
language:en
Short-container-title:

Author:

Laurent Vale´rie M.¹²,He´non Sylvie³,Planus Emmanuelle⁴,Fodil Redouane⁴,Balland Martial³,Isabey Daniel¹,Gallet Franc¸ois³

Affiliation:

1. INSERM Unite´ 492, Physiopathologie et The´rapeutique Respiratoires, Faculte´ de Me´decine et Faculte´ des Sciences et Technologie, Universite´ Paris XII, 8, rue du Ge´ne´ral Sarrail, 94010 CRE´TEIL cedex France

2. CNRS, UMR-7057 associe´ aux Universite´s Paris VI et Paris VII, Laboratoire de Biorhe´ologie et d’Hydrodynamique Physicochimique, et Fe´de´ration de Recherche Matie`re et Syste`mes Complexes, 2 place Jussieu, 75251 PARIS cedex 5, France

3. CNRS, ESA-7057 associe´ aux Universite´s Paris VI et Paris VII, Laboratoire de Biorhe´ologie et d’Hydrodynamique Physicochimique, 2 place Jussieu, 75251 PARIS cedex 5, France

4. INSERM Unite´ 492, Physiopathologie et at The´rapeutique Respiratoires, Faculte´ de Me´decine et Faculte´ des Sciences et Technologie, Universite´ Paris XII, 8, rue du Ge´ne´ral Sarrail, 94010 CRE´TEIL cedex France

Abstract

We compare the measurements of viscoelastic properties of adherent alveolar epithelial cells by two micromanipulation techniques: (i) magnetic twisting cytometry and (ii) optical tweezers, using microbeads of same size and similarly attached to F-actin. The values of equivalent Young modulus E, derived from linear viscoelasticity theory, become consistent when the degree of bead immersion in the cell is taken into account. E-values are smaller in (i) than in (ii): ∼34–58 Pa vs ∼29–258 Pa, probably because higher stress in (i) reinforces nonlinearity and cellular plasticity. Otherwise, similar relaxation time constants, around 2 s, suggest similar dissipative mechanisms.

Publisher

ASME International

Subject

Physiology (medical),Biomedical Engineering

Link

http://asmedigitalcollection.asme.org/biomechanical/article-pdf/124/4/408/5514526/408_1.pdf

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