Characterization of IcaB protein Poly-N-acetylglucosamine (dPNAG) De-N-acetylase involved in Staphylococcus epidermidis biofilm formation

Abstract

Quorum sensing (QS) is type of behaviour coordinating bacterial cell-cell interaction involved in EPS production, cell motility, toxin secretion, biofilm. Biofilms forming multidrug-resistant Staphylococcus epidermidis (MRSE) are major threats to public health undergoing slime formation and bacteremia. Biofilms causes nosocomial infection, chronic skin damage, vaginal wall inflammation, gut infection, endocardium infection and stomach infection. One of the serious concern is biofilm formation, which helps to prevent antibiotics diffusion leading to decrease in bacterial metabolic activity mechanism. Methicillin-resistant biofilm forming S. epidermidis, RP62A (ATCC 35984) isolate is slime-producing isolate. RP62A isolates has been well characterized from molecular and infection studies revealing that plasmid (pEpi62) carries antibiotics resistance genes to penicillin, kanamycin, erythromycin and streptomycin. De-N-acetylation of poly-N-acetylglucosamine (dPNAG) is an extracellular exopolysaccharide for biofilm development. We have characterized IcaB gene and protein sequence involved in Staphylococcus epidermidis biofilm formation.