The Tumor Markers Tpa, Tps, TpaCyk and Cyfra 21–1 React Differently with the Keratins 8, 18 and 19

Abstract

The commercially available tumor marker tests TPA, TPS, TPACYK and CYFRA 21–1 react with simple epithelium keratins. From clinical studies it can be deduced that the pattern of keratin recognition must be different for each of these tests. We therefore studied the reactivity of the keratin fragment combinations K8/K18 and K8/K19 in the different tests and determined the reactivity of the corresponding soluble antibodies with purified keratin 8, 18 and 19 in immunoblots. TPS and CYFRA 21–1 were found to distinguish clearly between the keratin fragment combinations K8/K18 (TPS) and K8/K19 (CYFRA 21–1). TPA and TPACYK reacted with both combinations, however, with different intensities. On immunoblots the CYFRA 21–1 antibodies reacted exclusively with K19, whereas the antibodies of the other assays reacted with at least 2 of the keratins investigated.