Effects of fibrin glue as a three-dimensional scaffold in cultivated adult human retinal pigment epithelial cells

Author:

Balagholi Sahar1,Rezaei Kanavi Mozhgan1ORCID,Alizadeh Shaban2,Dabbaghi Rasul3,Karami Samira2,Kheiri Bahareh4,Daftarian Narsis4

Affiliation:

1. Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2. Department of Hematology, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran

3. Department of Hematology, Faculty of Medical sciences, Tarbiat Modares University, Tehran, Iran

4. Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

This study was conducted to examine morphological, genotypic, and phenotypic alterations occurring in cultured adult human retinal pigment epithelial cells when encapsulated with different concentrations of fibrin glue. Cultivated adult human retinal pigment epithelial cells were encapsulated with different concentrations of fibrin glue, namely FG1 (42 mg/dl), FG2 (84 mg/dl), FG3 (124 mg/dl), FG4 (210 mg/dl), followed by the evaluation of genetic and cytomorphological changes and protein expression. Cultured adult human retinal pigment epithelial cells showed dendritiform morphology during the early days of encapsulation with fibrin glue. Moreover, an increasing inhibitory effect on cell growth was observed with increasing concentrations of fibrin glue. At the transcriptional level, the expression of MMP2, PAX6, and ITGB1 in FG1-encapsulated cells was significantly higher than that in other treated groups; however, the expression of ACTA2 was lower in all fibrin glue-encapsulated groups compared to that in the controls. Immunocytochemistry showed that FG2-encapsulated cells expressed cytokeratin 8/18, RPE65, and ZO-1 proteins, but not PAX6. In conclusion, fibrin glue at a concentration of 84 mg/dl allows proper encapsulation of adult human retinal pigment epithelial cells, while preserving the morphometric, genotypic, and phenotypic features of the cells. This three-dimensional biopolymer can be considered a reliable vehicle for retinal pigment epithelium cell transplantation in cell-based therapies.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biomaterials

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