Affiliation:
1. Dipartimento di Scienze e Tecnologie Biomediche, Universitá di Milano, Via Olgettina 60, 20132 Milano, Italy
Abstract
We describe a new electrochemical method for the determination of erythrocyte acetylcholinesterase activity (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) activity, based on the measurements of pH variation due to release of acetic acid from acetylcholine. The major advantages of the differential pH procedure are simplicity, high reproducibility, no need for pre-treatment of samples, automatic correction of sample blanks, and speed and direct measurement of enzymatic reaction. The proposed methods are linear up to 7400 U/L at 30°C and correlate well with the manual spectrophotometric method of Ellman1 for plasma cholinesterase and for washed erythrocytes. We adapted the same technique for the determination of erythrocyte cholinesterase using whole blood as sample and quinidine sulphate as inhibitor of pseudocholinesterase.
Subject
Clinical Biochemistry,General Medicine
Cited by
15 articles.
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