A Direct Radioimmunoassay for the Determination of Adrenocorticotropic Hormone (ACTH) and a Clinical Evaluation-Reference-Cited by-同舟云学术

A Direct Radioimmunoassay for the Determination of Adrenocorticotropic Hormone (ACTH) and a Clinical Evaluation

Published:1985-05 Issue:3 Volume:22 Page:247-256
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Marts C J¹,Koppeschaar H P F¹,Veeman W¹,Thijssen J H H¹

Affiliation:

1. Department of Endocrinology, University Hospital, State University of Utrecht, Catharijnesingel 101, Utrecht, The Netherlands

Abstract

A direct radioimmunoassay for the determination of ACTH in a small volume of serum was developed using a commercially available antiserum and tracer. A single-stage radioimmunoassay with an incubation period of 4 days gave reliable and reproducible ACTH values. In the incubation medium 0·1% 2-mercaptoethanol was used as the antioxidant. During a 15-month period the assay has been used routinely in the laboratory. The coefficient of variation was less than 10% in the physiological and pathological range. A normal range of 12 to 60 ng/L was found. The reliability of the assay is demonstrated by results obtained in patients with disorders of the pituitary-adrenal axis.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456328502200306

Reference21 articles.

1. Radioimmunoassay of ACTH in plasma

2. Rapid radioimmunoassay for corticotropin in unextracted human plasma.

Cited by 30 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Prognostic factors for outcome after transsphenoidal hypophysectomy in dogs with pituitary-dependent hyperadrenocorticism;Journal of Neurosurgery;2007-10

2. Aspects of pharmacodynamics and biotransformation of the glucocorticoid resocortol butyrate;Journal of Veterinary Pharmacology and Therapeutics;2004-10

3. Plasma concentrations of ACTH precursors correlate with pituitary size and resistance to dexamethasone in dogs with pituitary-dependent hyperadrenocorticism;Domestic Animal Endocrinology;2002-06

4. Combined pituitary hormone deficiency in German shepherd dogs with dwarfism;Domestic Animal Endocrinology;2000-10

5. Opioid and cannabinoid receptor-mediated regulation of the increase in adrenocorticotropin hormone and corticosterone plasma concentrations induced by central administration of Δ9-tetrahydrocannabinol in rats;Brain Research;1999-08