Enamel Proteins: Biosynthesis and Chemistry-Reference-Cited by-同舟云学术

Enamel Proteins: Biosynthesis and Chemistry

Published:1979-02 Issue:2_suppl Volume:58 Page:765-772
ISSN:0022-0345
Container-title:Journal of Dental Research
language:en
Short-container-title:J Dent Res

Author:

Sasaki S.¹,Shimokawa H.¹

Affiliation:

1. Department of Biochemistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113, Japan

Abstract

Bovine ameloblast cell layer was incubated in 3H-proline containing medium and the incorporation of radioactivity into protein fractions of the cell and the medium was investigated by SDSpolyacrylamide gel electrophoresis.The results suggest that the ameloblasts synthesize and secrete a protein having a molecular weight of 25,000 daltons which is supposed to be the prototype of enamel proteins. This protein was degraded into dialyzable sizes by the cell extract. After a longer period of incubation another low molecular weight protein fraction appeared in the medium.Purification procedure with SDS gel filtration of the enamel protein is reported.

Publisher

SAGE Publications

Subject

General Dentistry

Link

http://journals.sagepub.com/doi/pdf/10.1177/00220345790580022801

Reference14 articles.

1. The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

2. The ultracentrifugal and free zone electrophoretic characterization of the neutral soluble proteins of embryonic bovine enamel

3. Mechanic, G.L., Katz, E.P., Glimcher, M.J.: Biochem Biophys Acta 133:97-113 (1967).

4. Fincham, A.G.: In: Tooth Enamel II (Fearnhead, R. W., Stack, M. V., eds) pp. 79-87. Bristol: John Wright & Sons, Ltd., 1971.

5. Mechanic, G.L. : In: Tooth Enamel II ( Fearnhead, R. W., Stack, M. V., eds) pp. 89-94. Bristol: John Wright & Sons,Ltd., 1971.

Cited by 28 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Amelogenesis: Transformation of a protein-mineral matrix into tooth enamel;Journal of Structural Biology;2021-12

2. Expression of Enamel Proteins in Rough Endoplasmic Reticulum and Golgi Complex in Human Dental Germs;International Journal of Morphology;2017-06

3. Effects of Fluoride on the Interactions between Amelogenin and Apatite Crystals;Journal of Dental Research;2008-01

4. Amelogenin post-secretory processing during biomineralization in the postnatal mouse molar tooth;Archives of Oral Biology;1991

5. The Effect of Fluoride on the Developing Mineralized Tissues;Journal of Dental Research;1990-02