Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana-Reference-Cited by-同舟云学术

Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana

Published:2002-05 Issue:3 Volume:14 Page:240-242
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Alvarado Juan Francisco¹,Dolz Gaby²,Herrero Marco V.²,McCluskey Brian³,Salman Mo⁴

Affiliation:

1. Posgrado Regional en Ciencias Veterinarias Tropicales

2. Programa de Investigación en Enfermedades Tropicales

3. Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica, the Centers for Epidemiology and Animal Health, Veterinary Serices, USDA, APHIS, Ft. Collins, CO 80521

4. Center of Veterinary Epidemiology and the Animal Disease Surveillance Systems, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft. Collins, CO 80523–1676

Abstract

A competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of antibodies against vesicular stomatitis virus New Jersey (VSV-NJ) and vesicular stomatitis virus Indiana (VSV-IN) was compared with the serum neutralization test (SNT) using 1,106 serum samples obtained from dairy cattle on sentinel study farms in the Poás region of Costa Rica. Kappa coefficients between the C-ELISA and the SNT were 0.8871 (95% confidence interval [CI]: 0.8587–0.9155) and 0.6912 (95% CI: 0.6246–0.7577) for the VSV-NJ and VSV-IN tests, respectively. These results indicate good to excellent agreement between the 2 tests under these conditions.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063870201400309

Reference9 articles.

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3. An enzyme-linked immunosorbent assay for the detection of vesicular stomatitis virus antigen

4. Construction and insect larval expression of recombinant vesicular stomatitis nucleocapsid protein and its use in competitive ELISA

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