Biocompatibility and cellular uptake mechanisms of poly(N-isopropylacrylamide) in different cells

Author:

Guo Zhong1,Li Sha1,Wang Changyong1,Xu Jiake2,Kirk Brett3,Wu Jianping3,Liu Zonghua1,Xue Wei14

Affiliation:

1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, China

2. School of Pathology and Laboratory Medicine, University of Western Australia, Perth, WA, Australia

3. Department of Mechanical Engineering, Curtin University, Bentley, WA, Australia

4. Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou, China

Abstract

Thermosensitive poly( N-isopropylacrylamide) is widely used in various biomedical applications including drug delivery systems, gene delivery systems, switching devices, sensors, and diagnostic assays. To promote these clinical applications, it is essential to have a comprehensive understanding of the biosafety of poly( N-isopropylacrylamide) and the interaction of poly( N-isopropylacrylamide) with different cell lines, which has little research until now. In this work, we evaluated the biocompatibility of poly( N-isopropylacrylamide) including cell viability, nitric oxide production, and apoptosis of macrophages RAW264.7, human bronchial epithelial cells, A549, and human umbilical vein endothelial cells in the presence of poly( N-isopropylacrylamide). We have also examined the cellular uptake mechanisms of poly( N-isopropylacrylamide) using endocytic inhibitors and insighted into the intracellular co-localization of poly( N-isopropylacrylamide) using confocal laser scanning microscope. The results showed that poly( N-isopropylacrylamide) had good biocompatibility and could be internalized by these cells. It is macropinocytosis that poly( N-isopropylacrylamide) could be internalized in RAW264.7 cells and caveolae-mediated endocytosis in human bronchial epithelial cells, A549, and human umbilical vein endothelial cells. In addition, we also evidenced that intracellular poly( N-isopropylacrylamide) was co-localized with lysosome. The study provided important information for the development and clinical applications of poly( N-isopropylacrylamide) in the biomedical field.

Publisher

SAGE Publications

Subject

Materials Chemistry,Polymers and Plastics,Biomaterials,Bioengineering

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3