Evaluation of the Antidiabetic Activities of the Fruit of Parquetina nigrescens (Afzel.) Bullock and In Silico Identification of Its Antidiabetic Agent

Author:

Ayoola Marcus D1,Ogundeko Yetunde B1,Obanleowo Temiloluwa D1,Omole Deborah O1,Chukwu Blessing N1,Faloye Kolade O2

Affiliation:

1. Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria

2. Department of Chemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria

Abstract

The study investigated the antidiabetic potentials of the fruit extract of Parquetina nigrescens with the aim of justifying its folkloric antidiabetic usage in some part of Nigeria. Acute toxicity test of the plant extract was assessed using Lorke’s method. Its antidiabetic activities were assayed in α-amylase, α-glucosidase, glucose, and streptozotocin-induced diabetic rats’ models at various doses with acarbose and glibenclamide (5 mg/kg) as positive controls. Molecular docking studies were performed to identify the antidiabetic constituent of the extract and elucidate its possible mechanism of action. The estimated median lethal dose (LD50) of the extract was above 5000 mg/kg. In the α-amylase, α-glucosidase study, the extract elicited concentration-dependent activity similar to acarbose. In the glucose-induced hyperglycaemic model, 200 mg/kg of the extract was the most effective dose with comparable ( P > .05) antihyperglycaemic activity to glibenclamide (5 mg kg) at 1 to 4 h. Also in the streptozotocin-induced diabetic rats model, 100 and 200 mg/kg of the extract gave comparable ( P > 0.05) activity on days 4 to 14 that were significantly better than that of glibenclamide on days 4 to 7. The n-hexane and ethylacetate fractions of the extract, both at 200 mg/kg were the most active with comparable activity to glibenclamide at all time points. The molecular docking studies identified isorhoifolin as the best binder against alpha amylase with binding energy (−9.1 kcal/mol), alpha glucosidase (−9.4 kcal/mol), sodium-glucose cotransporter-2 (−9.5 kcal/mol), peroxisome proliferator activated receptor gamma (−10.3 kcal/mol), 11β- Hydroxysteroid dehydrogenase (−10.8 kcal/mol), and dipeptidyl peptidase IV (−9.4 kcal/mol). The results of the antidiabetic study of P nigrescence fruit extract justified its usage in ethnomedicne in diabetes management.

Publisher

SAGE Publications

Reference46 articles.

1. Sofowora A. Medicinal Plants and Traditional Medicine I. Africa. 3rd ed. Spectrum Books Ltd.; 2008:4, 50.0-80, 117-133.

2. World Health Organization. Global Report on Diabetes. Published April 2016. https://iris.who.int/bitstream/handle/10665/204871/9789241565257_eng.pdf?sequence=1

3. Phytochemistry and medicinal plants

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