Revisión: Aplicación de las técnicas cromatográficas al estudio de triglicéridos y esteroles de la grasa de leche / Review: Application of chromatographic techniques to the study of triglycerides and sterols of milk fat

Abstract

The latest developments in analytical techniques, mainly chromatographic, for the study of triglycer ides (TG) and sterols in milk fat are revised. Gas chromatography (GC) with packed or short capillary columns has been used to separate TG according to their carbon numbers (CN) and can be applied to the detection of foreign fats in milk fat. Combined or hyphenated chromatographic techniques are indispensable in order to identify and quantify individual molecular species of TG: thin layer chro matography (TLC) or high performance liquid chromatography (HPLC) with Ag+ as prefraction stage, followed by supercritical fluid chromatography, GC with long capillary column, and HPLC with reverse phase and mass spectrometry as detector. These techniques, along with non-specific and/or specific hydrolysis procedures, have allowed a better knowledge of the positional distribution of the fatty acids within the molecules of TG. Concerning the study of the esterol fraction, the supression of the stages of fat extraction—separation of the insaponifiable and derivatization—previous to the analy sis by GC represents a considerable advance that should be in part attributed to the improvement of the chromatographic techniques.