Relationship between semen quality and seminal plasma cholesterol, triacylglycerols and proteins in the domestic cat

Author:

García María F12,Nuñez Favre Romina12,Stornelli María C2,Rearte Ramiro13,García Mitacek María C12,de la Sota Rodolfo L12,Stornelli María A2ORCID

Affiliation:

1. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz, Argentina

2. Animal Reproduction Service, Faculty of Veterinary Science, National University of La Plata, La Plata, Buenos Aires, Argentina

3. Epidemiology and Public Health, Faculty of Veterinary Science, National University of La Plata, La Plata, Buenos Aires, Argentina

Abstract

Objectives The current study aimed to evaluate the relationship between specific seminal plasma components – cholesterol (CHOL), triacylglycerols (TAG) and total protein (PROT) concentrations – and semen quality in cats. A further aim was to determine the relationship between specific seminal protein bands and semen quality. Methods Thirteen toms, 2–5 years of age, were included. Semen collection was performed by electroejaculation every 4 weeks. Fifty-eight ejaculates were assessed for motility, velocity, volume, sperm concentration, total sperm count, viability, acrosome integrity, plasma membrane integrity and sperm morphology. Samples were divided into two groups: good semen quality (GSQ) and poor semen quality (PSQ). After evaluation, seminal plasma was separated from the sperm by centrifugation and stored at −20°C. CHOL, TAG and PROT concentrations were then assessed and seminal plasma protein profile was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results Seminal plasma CHOL and TAG concentrations, motility, velocity, sperm concentration, total sperm count and sperm morphology were significantly higher in GSQ cats compared with PSQ cats ( P <0.01). Moreover, seminal plasma SDS-PAGE analysis showed an identifiable extra band exclusively in the GSQ group. Conclusions and relevance Data obtained in this study showed that seminal plasma CHOL and TAG concentrations and specific protein bands could be used to improve semen evaluation in toms. In this sense, the 14 kDa protein band could be a valuable marker for semen quality in the cat and should be further investigated. However, more studies are necessary to determine its relationship with fertility.

Funder

Universidad Nacional de La Plata

Publisher

SAGE Publications

Subject

Small Animals

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