Morphometric Study of Fetal Brain Transplants in the Insular Cortex and NGF Effects on Neuronal and Glial Development

Abstract

Homotopic grafts supplemented with nerve growth factor (NGF) speed the recovery from learning deficits observed following electrolytic lesions of the insular cortex in rats. NGF also reduces the time in which the activity of choline acetyltransferase (ChAT) is first detected inside the graft by histochemical techniques. It is not known whether this behavioral and biochemical recovery correlates with an advanced maturation of the cellular elements within the graft, presumably induced by NGF. To investigate the degree of maturation of neurons, glial cells and blood vessels in NGF-supplemented grafts, adult rats were lesioned electrolytically in the insular cortex, and homotopic embryonic grafts (E16) with or without NGF supplementation were transplanted into the lesion. Fifteen days post grafting, the rats were perfused and the brains stained using silver impregnation techniques. Our results showed that neuronal maturation, as evaluated through several morphometric parameters, was advanced in NGF-supplemented grafts when compared with other experimental groups. Furthermore, grafts supplemented with NGF also showed significant increases in the number of neurons, oligodendrocytes, astrocytes and blood vessels. These observations indicated that the addition of NGF to insular cortex grafts promoted the maturation of neuronal and glial elements within the graft. They also support the possibility that the advanced morphological maturation of insular cortex grafts supplemented with NGF underlies the accelerated functional and biochemical recovery of animals with lesions of the insular cortex.