The induction of lipid peroxidation during the acute oxidative stress response induced by intratracheal instillation of fine crystalline silica particles in rats

Author:

Fukui Hiroko12,Endoh Shigehisa3,Shichiri Mototada2,Ishida Noriko2,Hagihara Yoshihisa2,Yoshida Yasukazu4,Iwahashi Hitoshi1,Horie Masanori4

Affiliation:

1. United Graduate School of Agricultural Science, Gifu University, Gifu, Japan

2. Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan

3. Research Institute for Environmental Management Technology (EMTECH), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

4. Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, Japan

Abstract

Crystalline silica (SiO2) is an important material for industry but is considered potentially carcinogenic. Inhalation of a crystalline SiO2 aerosol may contribute to serious lung diseases. Crystalline SiO2 particles are commonly used as a positive control in toxicity assays of particulate materials (e.g. nanoparticles). Crystalline SiO2 induces oxidative stress resulting in lipid peroxidation, but the acute oxidative stress response in the lung is not well understood. Lipid peroxidation during the acute stage of oxidative stress after instillation of crystalline SiO2 into rats was examined by bronchoalveolar lavage fluid (BALF) analysis. The levels of 8-iso-prostaglandin F and hydroxyoctadecadienoic acid (HODE) in the BALF were measured using liquid chromatography coupled to quadrupole mass spectrometry. The concentration of the antioxidant protein heme oxygenase-1 (HO-1) in the BALF was determined using enzyme-linked immunosorbent assay. Intratracheal instillation of crystalline SiO2 increased the level of HODE and HO-1 in BALF at 24 h after administration. The levels of HODE and HO-1 returned to baseline at 72 h after instillation. Lactate dehydrogenase leakage was observed only after 1 h instillation. These results suggest that the contribution of oxidative stress to the pulmonary toxicity of crystalline SiO2 is minimal in the early acute stage after exposure.

Publisher

SAGE Publications

Subject

Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Toxicology

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