The antioxidant effects of vitamin A, C, and E on aflatoxin B1-induced oxidative stress in human lymphocytes

Abstract

The aim of this study was to investigate the possible protective role of vitamin A, C, and E on aflatoxin B1-induced in human lymphocytes using biochemical approaches. The control group received dimethyl sulfoxide, the second group of cultures were administered aflatoxin B1 (AFB1) at a dose of 5 μM. The other group of cultures were treated with AFB1+vitamin A (0.5 and 1.0 and 1.5 μM) and AFB1+vitamin C (25, 50, and 100 μM) and AFB1+vitamin E (40, 100, and 200 μM). The results of this experiment show that AFB1 significantly decreased the level of GSH and the activities of superoxide dismutase and GPx and increased level of malondialdehyde. Simultaneous supplementation with vitamin A, C, and E restored these parameters to that of normal range. In conclusion, vitamin A, C, and E exhibited protective effects in human lymphocytes by inhibiting AFB1-induced ROS generation.