New Biomarkers for Prediction of Disseminated Intravascular Coagulation in Patients With Sepsis

Author:

Wakabayashi Ichiro1,Mambo Naomi2,Ueda Takahiro3,Nonaka Daisuke4,Lee Lyang-Ja4,Tanaka Kenji4,Kotani Joji5

Affiliation:

1. Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Hyogo, Japan

2. Department of Emergency, Disaster and Critical Care Medicine, Hyogo College of Medicine, Hyogo, Japan

3. Department of Emergency and Critical Care Medicine, Kindai University Faculty of Medicine, Osaka-Sayama, Osaka, Japan

4. Membrane Protein and Ligand Analysis Center, Protosera Inc., Yodogawa-ku, Osaka, Japan

5. Department of Disaster and Emergency Medicine. Kobe University Graduate School of Medicine, Hyogo, Japan

Abstract

Complication of disseminated intravascular coagulation (DIC) is a determinant of the prognosis for patients with sepsis. The purpose of this study was to find DIC-related peptides in blood for prediction and early diagnosis of DIC in patients with sepsis. The participants were 20 patients with sepsis (age: 68.9 ± 11.4 years) and they were divided into 2 groups with (n = 8) and without (n = 12) a complication of DIC. Peptides in the serum of the patients were inclusively analyzed by a new method for peptidome analysis using a target plate, BLOTCHIP. By differential analysis of peptides in the blood from patients in the groups with and without DIC, we selected 13 mass spectrometry (MS) peaks as candidate marker peptides for prediction of DIC. By subsequent MS/MS structural analysis, 8 peptides were successfully identified as marker peptides for DIC in patients with sepsis. The peptides were fragments of serum amyloid A-2 protein, α2-HS-glycoprotein, fibrinogen α chain, fibrinogen β chain, serum albumin, collagen α1 (I) chain, collagen α1 (III) chain, and coagulation factor XIII A chain. In receiver–operating characteristic analysis for the relationships between the marker peptides and DIC, the area under the curve for each of these peptides was 0.594 to 0.760. We identified 8 blood marker peptides for prediction of DIC complication in patients with sepsis. Further studies by direct measurements of the serum peptide levels in larger numbers of patients with sepsis-induced DIC are needed to confirm the findings of this study.

Funder

Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science

Publisher

SAGE Publications

Subject

Hematology,General Medicine

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