Five New Ent-Atisene Glycosides From Stevia rebaudiana

Abstract

Five new ent-atisene diterpene glycosides (1-5) have been isolated from Stevia rebaudiana Bertoni. This adds to a list of only 2 new ent-atisene diterpene glycosides isolated previously from S. rebaudiana. Diterpene glycosides isolated from S. rebaudiana, such as the rebaudiosides, are typically associated with the ent-kaurene core and glycosylation at the C-13 and C-19 positions. Extensive application of nuclear magnetic resonance spectroscopy techniques (1H, 13C, 1D-TOCSY, COSY, HSQC-DEPT, HMBC, and ROESY) as well as high-resolution mass spectrometry demonstrated that the ent-atisenes 1 to 5 had glycosylation patterns identical to rebaudiosides J, N, O, K, and T, respectively. We have named these compounds stevatisene J, N, O, K, and T, respectively.