Synthesis of Oligosaccharide Libraries Through Glycosylations on Unprotected Acceptors

Abstract

Starting from an unprotected GlcNAc acceptor, after first random glycosylation and deprotection, and second random glycosylation, deprotection, and purification, we were able to obtain a trisaccharide library containing 36 α-linked trisaccharide library. These compounds were characterized by 1D, 2D NMR and mass spectra. The library was used as the starting material for enzymatic reaction to obtain a tetrasaccharide in support of the results. Based on this method, a trisaccharide library containing 15 α-linked trisaccharides was synthesized.