Affiliation:
1. Department of Cellular and Molecular Biology, Harvard University, 16 Divinity Avenue. Cambridge, Massachusetts 02138
Abstract
The expression of circadian rhythmicity in Gonyaulax polyedra is strikingly altered by an inhibitor of protein phosphorylation. The effects of 6-dimethylaminopurine (6-DMAP), known to reversibly block cell division in many systems through inhibition of protein kinase activity, are described here for Gonyaulax. Its action appears to be exclusively tonic in nature; in cells continuously exposed, the period is lengthened in a concentration-dependent fashion. Shorter treatments at a higher concentra tion of 6-DMAP (5 mM) apparently stop the circadian oscillator, but reversibly so, since the rhythm resumes after drug removal with a phase delay approximately equal to the duration of the treatment. Pulses of the inhibitor are effective in causing phase delays at all times of the circadian cycle. In addition, 6-DMAP completely blocks light-induced phase advances and is effective in inhibiting many Gonyaulax protein kinases in vitro.
Subject
Physiology (medical),Physiology
Cited by
44 articles.
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