Mechanism of Action of Clove and Ginger Herbal Formulation-Mediated TiO2 Nanoparticles Against Lactobacillus Species: An In Vitro Study

Abstract

Introduction: With its numerous practical applications, the usage of nanotechnology in the medical profession has been a godsend in our modern technological era. Titanium dioxide (TiO2) is a semiconducting metal oxide with superior inherent qualities which can be utilized in various applications across various fields. Taking into account all of the above information, the current study focused on the mechanism of action of TiO2 nanoparticles mediated by clove and ginger herbal formulations against Lactobacillus species. Materials and Methods: Extract of clove and ginger herbal formulation-mediated TiO2 nanoparticles was obtained with acetone in the ratio of 10:1, yielding 9 mg/mL. After overnight incubation and further serial dilutions, the solution was introduced into microplate wells with cultured Lactobacillus species for 4 hours. A 5% of test solution was added into Kimble tubes containing Muller-Hinton broth along with the plant extract, followed by recording of minimum inhibitory concentrations at certain time intervals. The number of samples used for each concentration was 4 using the convenience method. The grouping sample was 3, i.e. test group, antibiotic and positive control group. The results were statistically analysed following one-way analysis of variances using SPSS software version 22. Result: Optical density determines the degree of scattering of light that is produced by a bacterium within a culture plate. The more the bacteria, the more the light is scattered. The results ( P < .005) show that as the concentration increases, the value of optical density decreases which proves that there occurs a bactericidal process that results in the reduced bacterial count. Conclusion: The results of this study suggest that TiO2 nanoparticles enhanced with clove and ginger might be used as an antibacterial agent against Lactobacillus species.