A murine model with aspects of clinical relevance for the study of antibiotic-induced endotoxin release in septic hosts

Abstract

A murine model which utilizes a burn to increase susceptibility to sepsis is presented. The model is inexpensive, highly reproducible, versatile, easy to use, and clinically relevant to many aspects of a Gram-negative sepsis which develops over time from a localized focus of infection. Additionally, studies of LPS-intoxication in mice with the same burn indicate that the animals show increased sensitivity to endotoxin without having to be exposed to exogenous sensitizing chemicals. Hence, this burned mouse model of sepsis might provide a sensitive, clinically relevant model for the study of antibiotic-induced endotoxin release from Gram-negative bacteria in septic hosts.