Protein a Carrying PMMA Microbeads: Adsorption of Cholesterol and HlgG from Human Plasma

Abstract

Cholesterol and HlgG adsorbed from human plasma obtained from a hypercholesterolemia patient, onto protein A-immobilized polymethyl-methacrylate uniform microbeads carrying different amounts of protein A (0.264-1.682 mg protein A/g PMMA, or 0.66-4.2 mg protein A/m2PMMA) were investigated in batchwise experiments. There was no interaction between protein A molecules and cholesterol when cholesterol aqueous solutions were used. However, there was significant cholesterol and HlgG adsorption from the plasma obtained from a patient with hypercholesterolemia. The maximum amounts of cholesterol and HlgG adsorbed were 3.96 μmol cholesterol/g PMMA (5.4 mg cholesterol/g PMMA) and 0.242 μmol IgG/g PMMA (35.4 mg IgG/g PMMA).