Development of a Vibratory Microinjection Method

Author:

Miyawaki F.1,Arai Y.2,Morisaki T.2,Ahmed S.1,Omata S.3,Fukui Y.1

Affiliation:

1. College of Science and Engineering, Tokyo Denki University - Japan

2. Department of Bioscience, National Cardiovascular Center Research Institute - Japan

3. Department of Electrical and Electronic Engineering, Nihon University - Japan

Abstract

To reduce cellular damage by pronuclear microinjection and nuclear transfer, we have recently developed a vibratory microinjection method. A micropipette was fixed to a piezoelectric ceramic with a resonance frequency of 70 kHz. When this micropipette was vibrated, it easily entered a mouse-fertilized egg without any sharp depression of the cell body, whereas a sharp, deep depression at the insertion site was observed when the micropipette was not vibrated. A depression rate defined as a rate of a depth of depression over an original cell diameter was utilized as an index of cellular deformation. The depression rates with and without vibration were 11.1 ± 5.2% (N = 24) and 40.4 ± 8.8% (N = 16), respectively (P < 0.0001, Student's t-test). In conclusion, the vibratory microinjection method is a new, useful option for gene transfer because it resulted in much less cellular deformation, therefore implicating less cellular damage.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biomaterials,General Medicine,Medicine (miscellaneous),Bioengineering

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