Trichinella Spiralis-Ab3 (IgG, IgM, IgA), IL-6 and Tnf Levels in Murine Trichinellosis

Abstract

The syngeneic anti-anti-idiotypic antibodies (Ab3) IgG, -IgM, and -IgA induced by polyclonal mouse anti-idiotypic antibodies (Ab2) against the rabbit F(ab) T. spiralis-Ab1, was studied. IL-6 level and cytotoxic activity (TNFα and LT) in the serum of mice infected with L1- T. spiralis or immunized with T. spiralis-Ab2 antibodies were also determined. The Ab1 antibody response was found to reflect the life cycle of the parasite. High IgA-, IgM-, and IgG-Ab1 levels, as well as corresponding levels of cytotoxic activity (62.3, 88.8 and 107.8 5/ml) and IL-6 (216, 447 and 789 U/ml) on days 10, 20 and 28 after the infection, respectivelly, were detected. The Ab3 antibody response against the polyclonal T. spiralis-Ab2, followed the classical pattern of antibody level after initial infection with an immunogen. IgM was first detected 10 days p.i., while IgA and IgG were detected 20 days p.i. Cytotoxic activity of 93.2, 245.9, and 326.7 U/ml, and IL-6 levels of 237, 304, and 487 U/ml, were detected on 10, 20, and 28 days p.i., respectivelly. Although Ab3 bound to T. spiralis-Ab2 antibodies or to L1 -T-spiralis antigen and cytotoxic activity in mice immunized with T. spiralis-Ab2 antibodies was increased, there was no protection against the parasitic infection.