Affiliation:
1. School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, PR China
Abstract
Aim: The metabolism of tryptophan (TRP) through kynurenine (KYN) and 5-hydroxytryptamine (5-HT) pathways is linked to various diseases such as neurological diseases and cancer. The levels of 5-HT, KYN, TRP can be used as indicators for the diagnosis of various diseases in clinical and scientific research. Experimental: Since 5-HT, KYN, TRP are all endogenous molecules in biological samples, it is difficult to obtain a ‘real blank sample’. A surrogate analyte-based LC–MS/MS method was chosen, using 5-HT-d4, KYN-d4 and TRP-d5 as surrogate analytes to replace the authentic analytes 5-HT, KYN and TRP, respectively. Theophylline was selected as the internal standard (IS). Results: The method was applied to quantification of 5-HT, KYN and TRP of plasma, liver, colon, brain and verified to be acceptable in terms of linearity, precision, accuracy, matrix effect, recovery efficiency and stability.
Subject
Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry
Cited by
8 articles.
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