A novel approach for quantitation of glucosylceramide in human dried blood spot using LC

A novel approach for quantitation of glucosylceramide in human dried blood spot using LC–MS/MS

Published:2015-07 Issue:12 Volume:7 Page:1483-1496
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Ji Allena Ji¹,Wang Haixing¹,Ziso-Qejvanaj Enida¹,Zheng Kefei¹,Chung Lee Lee¹,Foley Timothy¹,Chuang Wei-Lien²,Richards Susan¹,Sung Crystal¹

Affiliation:

1. Clinical Specialty Laboratory, DSAR, Sanofi, 1 The Mountain Road, Framingham, MA 01701, USA

2. Biological Mass Spectrometry & Biomarker Research, Sanofi, 1 The Mountain Road, Framingham, MA 01701, USA

Abstract

Background: Glucosylceramide, an efficacy biomarker for Gaucher Type 1 disease, exhibits poor solubility in polar solvents and whole blood which makes it difficult to prepare a homogenous blood standard. Results: We developed a novel method using standard addition approach by spiking a small volume of analyte solution on the surface of prespotted dried blood spot. The whole spots were punched out for subsequent extraction and LC–MS/MS analysis. The assay performance met all validation acceptance criteria. Glucosylceramide concentrations in 50 paired plasma and dry blood spot samples obtained from Gaucher Type 1 patients were tested and the results demonstrated the feasibility of using the DBS method for clinical biomarker monitoring. Conclusion: The new approach greatly improves assay precision and accuracy.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.15.77

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