Quantification of 18 amino acids in human plasma: application in renal transplant patient plasma by targeted UHPLC

Quantification of 18 amino acids in human plasma: application in renal transplant patient plasma by targeted UHPLC–MS/MS

Published:2016-07 Issue:13 Volume:8 Page:1337-1351
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Wang Qinghua¹²,Wen Yan¹,Xia Tianyi¹,Xiong Xiaojuan²,Gao Shouhong¹,You Chunhua¹²,Tao Xia¹,Zhang Feng¹,Chen Wansheng¹

Affiliation:

1. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China

2. Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Bioengineering Research Institute, Yichun University, Yichun 336000, PR China

Abstract

Aim: Quantification of amino acids in human plasma has become an important and essential analysis parameter in life science. In this paper, we developed a targeted UHPLC–MS/MS method for 18 amino acids in the renal transplant patients. Methods & results: Plasma in small volume (150 μl) was pretreated by a one-step protein precipitant extraction for analysis. Detection was executed by MS/MS in the MRM mode. Assays were validated according to current bioanalytical guidelines, with good linearity (R > 0.99), intraday and interday precision (CV < 11.6%, RE ≤ ± 14.8%), extraction recovery (between 77.4 and 117.6%), matrix effect (73.3–118.0%) and stability (RE≤ ±14.7%). Conclusion: The method was successfully applicable for amino acid analysis in the renal transplant patient.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio-2016-0057

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