Zebrafish live imaging reveals only around 2% rather than 50% of motor neurons die through apoptosis during development

Author:

Jia Hao1,Yang Hongmei1,Luo Kathy Qian12

Affiliation:

1. Faculty of Health Sciences, University of Macau

2. Ministry of Education Frontiers Science Center for Precision Oncology, University of Macau

Abstract

It is widely accepted that neurons will die through apoptosis if they cannot receive enough growth factors during development of vertebrates; however, there is still no real-time observation showing this dying process in live animals. Here, we generated sensor zebrafish achieving live imaging of motor neuron apoptosis at single-cell resolution. Using these sensor zebrafish, we observed for the first time that in an apoptotic motor neuron, caspase-3 activation occurred quickly within 5-6 min and at the same time between the cell body and axon. Interestingly, we found that only around 2% of motor neurons died during zebrafish development, which is much lower than the generally believed 50% cell death occurred in embryonic stage of vertebrates. Our data also showed that most of the apoptotic bodies of these dead motor neurons were not cleared by macrophages. These sensor zebrafish can serve as powerful tools to study motor neuron apoptosis in vivo .

Publisher

eLife Sciences Publications, Ltd

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3