Using and Validation of a new Spectrophotometric method for determination of Vitamin C in Pharmaceutical samples-Reference-Cited by-同舟云学术

Using and Validation of a new Spectrophotometric method for determination of Vitamin C in Pharmaceutical samples

Published:2023-04-29 Issue: Volume: Page:1731-1737
ISSN:0974-360X
Container-title:Research Journal of Pharmacy and Technology
language:en
Short-container-title:RJPT

Author:

Tayar Mary¹,Mohammad Haroon¹,Moazeen Lujen²

Affiliation:

1. Department of Pharmaceutical Chemistry and Drug Quality Control, Faculty of Pharmacy, Tishreen University, Lattakia, Syria.

2. Department of Applied Chemistry, Faculty of Science, Damascus University, Damascus, Syria.

Abstract

A new simple and rapid spectrophotometric method for determination the accurate amount of vitamin C containedin pharmaceutical samples has been developed. The ferric chloride is reduced by vitamin C and then reacts with potassium ferricyanide to create a dark blue solution of Prussian blue. The resulted color was observant at the absorption wavelength of 697nm. Optimum reaction conditions of Ferric chloride, Potassium Ferricyanide concentrations, reaction time, temperature and pH where investigated. Influence of potential interfering substances were also examined.Analyzes of commercial pharmaceutical samples showed good correlation with the valuesgiven by the manufacturers and the results obtained by a titration method with Iodine. The apparent molar absorptivity value is found to be 3.25 × 103. The results of analysis have been validated statistically, with good correlation coefficient (0.999). The proposed method has better LOD and LOQ values than many methods used to determine vitamin C in pharmaceutical forms.

Publisher

A and V Publications

Subject

Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Reference38 articles.

1. Naidu, KA. Vitamin C in Human Health and Disease is still a mystery. An overview. Nutrition journal, 2003; 2(1): p. 1-10.

2. Salkić, M. and A. Selimović. Spectrophotometric determination of L-ascorbic acid in pharmaceuticals based on its oxidation by potassium peroxymonosulfate and hydrogen peroxide. Croatica Chemica Acta. 2015; 88(1): p. 73-79://dx.doi.org/10.5562/cca2551.

3. Fujita Y, Mori I, Yamaguchi T, Hoshino M, Shigemura Y, Shimano M. Spectrophotometric determination of ascorbic acid with iron (III) and p-carboxyphenylfluorone in a cationic surfactant micellar medium. Analytical Sciences. 2001; 17(7): p. 853-857. https://doi.org/10.2116/analsci.17.853.

4. Klepo L, Copra-Janicijevic A, and Kukoc-Modun L. A new indirect spectrofluorimetric method for determination of ascorbic acid with 2, 4, 6-Tripyridyl-S-Triazine in pharmaceutical samples. Molecules. 2016; 21(1): p. 101. https://doi.org/10.3390/molecules21010101.

5. Teepoo S , Chumsaeng P, Jongjinakool S , Chantu K and Nolykad W. A new simple and rapid colorimetric screening test for semi-qualitative analysis of vitamin C in fruit juices based on Prussian blue. Journal of Applied Sciences. 2012; 12(6): p. 568-574. DOI : 10.3923/jas.2012.568.574.