In vitro Determination of SPF for Syrian Olive (Olea europaea) Leaf Extracts

Abstract

Over exposure to sun radiation can cause a lot of skin disorders like erythema, hyperpigmentation, photoaging and cancers. In recent years, there has been an increasing interest in plants which are rich in bioactive compounds. These compounds have a lot of benefit toward cancers, cardiovascular diseases, aging etc. Among these compounds are polyphenols and their derivatives. This study showed several methods of extraction phenolic compounds from different cultivars of olive leaves using water or ethanol 80% (v/v) as solvents in different condition. The content of oleuropein compound was determined using HPLC method. Then using IR to emphasize the structure of the compounds in olive leaves. Ethanolic extract using Soxhlet apparatus was high in oleuropein. Oleuropein amount as a main phenolic compound in olive leaves was 2-7%. Then in vitro SPF number for olive leaves was determined according to the spectrophotometric method described by Mansur et al and the values were 14-29.9 in 1mg/ml.