Molecular identification of Streptomyces sp. isolated from peat land of Palangka Raya, Kalimantan Tengah using 16S rRNA gene sequences analysis

Abstract

Bacterial identification with 16S rRNA accompanied with cloning techniques to obtain a purer sequence are now highly developed. This research aims to identify Streptomyces bacteria from peatlands in Central Kalimantan as a source of new antibiotics compound using 16srRNA and cloning techniques. Streptomyces sp. isolate was studied in detail and characterized. The morphological and biochemical characterization and tentative identification methods was described following the Bergey’s Manual of Systematic Bacteriology. Further identification and phylogenetic study were done using molecular tools. 16S rRNA helped to determine the species using PCR. Results of tabulation data from BLAST® indicate that isolates 2.1 belong to the genus Streptomyces sp. with the percentage of identity reaching 99.73% with Streptomyces sp. VEL 17 gene for 16S rRNA, partial sequences.