Microflora of hatching eggs and ways of its formation-Reference-Cited by-同舟云学术

Microflora of hatching eggs and ways of its formation

Published:2023-09-29 Issue: Volume: Page:
ISSN:2713-1602
Container-title:Pharmacy Formulas
language:
Short-container-title:Pharmacy Formulas

Author:

Gorfunkel Elena P.,Ilina Larisa A.

Abstract

In this study, the results were obtained reflecting the content of the genetic material of bacteria in incubation eggs. Given the widespread use of chicken embryos in medicine, veterinary medicine and pharmacology, the identification and identification of microorganisms is an important task. In the course of the study, a quantitative and qualitative analysis of the composition of bacteria in various tissues of incubation eggs was carried out. Three average mixed samples of each typical category were selected: chorion-allantois shell (HAO) on the 8th day of incubation, yolk sacs on the 13th, 18th and 20th days of incubation. The study was conducted using real-time PCR (real-time PCR). Sampling and sample preparation were carried out in accordance with established standards. The data obtained are important to take into account when using incubation eggs as an experimental model or for obtaining biological products. As a result of this study, the content of bacterial genetic material in various tissues of incubation eggs was established, which is important for assessing the safety and quality of these eggs when they are used in medical, veterinary and pharmacological studies. The real-time PCR method made it possible to conduct a quantitative and qualitative analysis of the composition of bacteria, which is an important step in studying the identification of microorganisms in incubation eggs. The established rules and standards for sampling and sample preparation ensure the reliability and accuracy of the data obtained.

Publisher

ECO-Vector LLC

Subject

Materials Science (miscellaneous)

Reference8 articles.

1. Shmarov M.M., Logunov D.Yu., Cherenova L.V., Picker E.G. Technology for producing recombinant CELO adenoviruses in chicken embryos in preparative quantities // Biomedicine. - 200 - No. 1 - P. 112-113. (In Russ).

2. Stroganova, I.Ya. Chicken embryos and their use in virology: method. instructions for laboratory classes / I.Ya. Stroganov; Krasnoyarsk State Agrarian University. – Krasnoyarsk, 2013. – 19 p. (In Russ).

3. Monstakova T.V., Azarnova T.O., Kochish I.I. Prospects and advantages of using chicken embryos as model systems for pharmaceutical research using the example of cobalt glycine // Veterinary Pharmacological Bulletin - 2021 - No. 2 - P.84-91. (In Russ).

4. APPROPRIATE CONDITIONS FOR CULTIVATING THE STRAIN OF NEWCASTLE DISEASE VIRUS IN THE DEVELOPING HEN EMBRYOS

5. Knyazeva, V. A. Study of the influence of a magnetic field and laser radiation on the development of the musculoskeletal system of a chicken embryo / V. A. Knyazeva, A. G. Shutenkov; scientific hands F. I. Suleymanov // Youth - science and practice of the agro-industrial complex: materials of the 102nd International Scientific and Practical Conference of Students and Postgraduate Students, Vitebsk, May 29-30, 2017 / Vitebsk State Academy of Veterinary Medicine. - Vitebsk: VGAVM, 2017. - Part 1: Veterinary medicine and biological sciences. - P. 179(In Russ).