Combination of rice blast resistance genes in the genotypes of russian rice varieties with the use of marker assisted selection

Abstract

Grain productivity of rice is significantly reduced by dangerous disease – blast. Therefore, the development of resistant high yielding rice varieties with the Pi group of genes is important. Use of molecular markers linked with the loci of resistance significantly optimizes the breeding process. The purpose of the research is to develop rice lines combining 2-6 loci of resistance to blast: Pi-1, Pi-2, Pi-33, Pi-ta, Pi-b, Pi-40 by molecular marking. Materials and Methods. As donors of resistance genes foreign samples were used, recipient – Russian varieties. In the studies we used micro-satellites markers and PCR analysis. Results. In the first stage of the research as a result of hybridization domestic lines with genes Pi-l, Pi-2, Pi-33 were obtained. At the second stage – hybrids with all 3 genes were developed. In the third stage genes Pi-b and Pi-ta and on the fourth – Pi-40 were introduced. Conclusion. As a result, rice genotypes, combining 6 loci of blast resistance were developed with use of marker assisted selection.