Development of the method for spectrophotometric quantitative determination of the total flavonoids in <i>Bupleurum multinerve</i> herb-Reference-Cited by-同舟云学术

Development of the method for spectrophotometric quantitative determination of the total flavonoids in Bupleurum multinerve herb

Published:2020-07-15 Issue:5-6 Volume:20 Page:170-174
ISSN:2410-3764
Container-title:Aspirantskiy Vestnik Povolzhiya
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Short-container-title:Aspirantskiy Vestnik Povolzhiya

Author:

Petukhova Svetlana A.,Posokhina Alina A.,Mirovich Vera M.

Abstract

The article presents the method for the quantitative determination of flavonoids for the analysis of Bupleurum multinerve herb based on the method of differential spectrophotometry. The optimal conditions for analysis have been determined. They include extractant 40% ethyl alcohol, ratio of raw materials and extractant 1 : 100, extraction time of 60 minutes in the boiling water bath, complexing agent of 1 ml of 2% aluminum chloride. The use of rutin as a standard has been experimentally validated, analytical wavelength is 412 nm. The relative error of the mean result (for n = 9) was 3.20%. Validation studies of the method have shown that it meets the criteria: linearity (r = 0.99988), correctness, specificity, and precision. The analytical range of the method is 8.6726.08 g/ml. The method is recommended for the inclusion into the new edition of the Pharmacopoeia Monograph for this type of plant material.

Publisher

ECO-Vector LLC

Link

https://journals.eco-vector.com/2410-3764/article/viewFile/42337/pdf

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