The Investigation of the Effects of Different Seminal Plasma Heating Processes on Short Term Storage of the Akkaraman Kangal Ram Sperm

Abstract

This research, seminal plasma in sperm was examined to determine the effect on sperm and to evaluate the use of reproductive purposes. For the research, the sperm obtained from 24-36 months of age 8 rams were used. Sperm was collected with the help of an electro-ejaculator out of mating season. The sperm collected were divided into 3 equal volumes after combining. It was kept to process in a 28 ⁰C water bath. The sperm were separated from the seminal plasma by centrifugation and combined with the sperm that is separated from after it was kept in 60 ⁰C and 80 ⁰C for 10 minutes and separated again in 28 ⁰C medium. The untreated control group and 60 ⁰C and 80 ⁰Cgroups were kept in a 4 ⁰C environment. Motility, live/dead ratio, and sperm abnormalities were recorded in 3 groups that were controlled once every 8 hours. According to the results of the study, when the control group and experimental groups were compared, the group with the longest duration of motility was determined as 80 ⁰C groups. Results of this study prove that, the denaturation of various proteins and enzymes in the seminal plasma of sperm directly proportional to lifetime, abnormalities, and motility.