Author:
Lopez Diego,Bischof Jesse
Abstract
Metal-analyte interaction is a type of nonspecific adsorption (NSA) that occurs mainly between acidic analytes and active adsorptive sites present in the metal surfaces of the fluidic path of any chromatographic system. NSA represents a chromatographic challenge since it may lead to poor peak shape, low recovery, and poor performance from the high performance liquid chromatography (HPLC) system. As a result, many solutions that circumvent and mitigate this type of secondary interaction have been published or commercialized in the last few years. Such solutions encompass swapping stainless steel components for non-reactive polymers, “biocompatible” metals or alloys, the addition of additives into the mobile phase, pretreating the system with large amounts of sample or strong acids, and the use of coated stainless steel materials. This article explores these solutions in more detail, particularly the recent adaptation and benefits of coated metal surfaces in LC.
Publisher
Multimedia Pharma Sciences, LLC
Reference37 articles.
1. Collins, K. E.; Collins, C. H.; Bertran, C. A. Stainless Steel Surfaces in LC Systems, Part II—Passivation and Practical Recommendations. LCGC North Am. 2000, 18 (7), 688–692.
2. Hsiao, J. J.; Staples, G. O.; Stoll, D. R. Troubleshooting LC Separations of Biomolecules, Part 1: Background, and the Meaning of Inertness. LCGC Eur. 2020, 33 (3), 122–126.
3. Euerby, M. R.; Johnson, C. M.; Rushin, I. D.; Sakunthala Tennekoon, D. A. S. Investigations into the Epimerisation of Tipredane Ethylsulphoxide Diastereoisomers during Chromatographic Analysis on Reversed-Phase Silica II. The Involvement of Metals in Commercially Available C18 Silicas. J. Chromatogr. A 1995, 705 (2), 229–245. DOI: 10.1016/0021-9673(95)00273-P
4. De Pra, M.; Greco, G.; Krajewski, M. P.; et al. Effects of Titanium Contamination Caused by Iron-Free High-Performance Liquid Chromatography Systems on Peak Shape and Retention of Drugs with Chelating Properties. J. Chromatogr. A 2020, 1611, 460619. DOI: 10.1016/j.chroma.2019.460619
5. DeLano, M.; Walter, T. H.; Lauber, M. A.; et al. Using Hybrid Organic–Inorganic Surface Technology to Mitigate Analyte Interactions with Metal Surfaces in UHPLC. Anal. Chem. 2021, 93 (14), 5773–5781. DOI: 10.1021/acs.analchem.0c05203
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献