Abstract
The gradient delay volume is one of the most important, yet least understood, parameters that affect how gradient elution separations in liquid chromatography (LC) work. This parameter has implications for method development and method transfer during the lifecycle of an LC method. In this installment, Dwight R. Stoll illustrates the impact of different gradient delay volumes when transferring a method between instruments and discuss some strategies that can be used to mitigate these challenges.
Publisher
Multimedia Pharma Sciences, LLC