A Turn-on Fluorescent Sensor Based on Copper-Based Metal-Organic Frameworks for Sensitive Detection of L-Histidine

Author:

Chu Chengxiang1ORCID,Huang Rui12,Wang Xianfeng1,Deng Yuanyi1,Gu Tao1,Huo Danqun1,Zhang Liang12,Zhong Daidi1,Hou Changjun13

Affiliation:

1. Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, P. R. China

2. National Engineering Research Center of Solid-State Brewing, Luzhou Laojiao Group Co., Ltd., Luzhou 646000, P. R. China

3. Chongqing Key Laboratory of Bio-Perception & Intelligent Information, Processings School of Microelectronics and Communication Engineering, Chongqing University, Chongqing 400044, P. R. China

Abstract

As an important amino acid in the body, L-histidine participates in various physiological processes. Therefore, it is of great necessity to establish a rapid and effective method for L-histidine sensing, especially in blood and urine. In this work, a fluorescence sensor for detection of L-histidine was successfully developed using Cu-MOFs/Fe[Formula: see text] sensor. Cu-MOFs with blue emission served as the identification unit and signal source, and Fe[Formula: see text] played the role of “quencher” in this system. Fe[Formula: see text] induced the fluorescence quenching of Cu-MOFs to form Cu-MOFs/Fe[Formula: see text] sensor due to the effect of photoinduced electron transfer (PET). After the addition of L-histidine, Fe[Formula: see text] was captured by L-histidine to form L-histidine-Fe[Formula: see text] complex, which inhibited the PET effect from Cu-MOFs to Fe[Formula: see text], resulting in the fluorescence recovery of Cu-MOFs. With such a design, L-histidine detection was realized with a wide linear range from 1[Formula: see text][Formula: see text]M to 190[Formula: see text][Formula: see text]M. The limit of detection (LOD) was calculated as low as 0.156[Formula: see text][Formula: see text]M. In addition, successful detection of L-histidine in blood serum with high selectivity and sensitivity indicated the real applications and potential diagnostic value of Cu-MOFs/Fe[Formula: see text] system.

Funder

National Natural Science Foundation of China

Fundamental Research Funds for the Central Universities

Chongqing Municipal Training Program of Innovation and Entrepreneurship for Undergraduates

Graduate Scientific Research and Innovation Foundation of Chongqing, China

The Higher Education Teaching Reform Research Project of Chongqing

Publisher

World Scientific Pub Co Pte Lt

Subject

Condensed Matter Physics,General Materials Science

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