Applying Principles of Multi-Factor Genetic Analysis of Infectious Disease Agents in the Work of the Rospotrebnadzor SAET during Mass Events
-
Published:2018-07-04
Issue:2
Volume:
Page:68-72
-
ISSN:0370-1069
-
Container-title:Problems of Particularly Dangerous Infections
-
language:
-
Short-container-title:Problemy Osobo Opasnykh Infektsii
Author:
Kuznetsova I. V.,Efremenko D. V.,Kulichenko A. N.
Abstract
Objective of the study – to develop and test in operation of the Rospotrebnadzor Spesialized Anti-Epidemic Teams (SAET), deployed during the major mass events, the procedure for application of molecular-genetic methods for detection and identification of pathogenic biological agents. Materials and methods. Utilized are the reporting documentation drawn up by the results of work of the specialized anti-epidemic teams of the Rospotrebnadzor on the provision of sanitary epidemiological welfare during Olympic Games 2014 and other important mass events in Sochi in 2015–2017. Results and conclusions. Developed and tested in practice of the Rospotrebnadzor SAET during the major mass events has been procedure for application of molecular-genetic methods, based on structured approach (algorithm) of indication and genetic characterization of PBA. Equipping of the SAET with diagnostic preparations is carried out in accordance with the provision of preparedness for performing investigations by three-level algorithm of molecular-genetic characteristics of pathogens: level I – detection (indication) of a pathogen using PCR, level II – identification of the pathogen genome fragments using PCR, level III – genotyping. Developed procedure was used successfully by the SAET during the Olympic Games 2014 and other important mass events in Sochi in 2015–2017, which allows for recommending it for future use.
Publisher
Russian Research Anti-Plague Institute Microbe
Subject
Infectious Diseases,Microbiology (medical),Immunology,Microbiology,Epidemiology
Reference14 articles.
1. Balakhonov S.V., Andaev E.I., Chesnokova M.V., Allenov A.V., Khomenko T.V., Ivanov L.I., Nikitin A.Ya., Kosilko S.A., Koval’sky A.G., Kulikalova E.S. [The role of anti-plague institutions in provision of the epidemiological well-being during the preparation and carrying out of the APEC 2012 Summit]. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections]. 2013; 3:5–12. DOI: 10.21055/0370-1069-2013-3-5-12. 2. Ezhlova E.B., Demina Yu.V., Kulichenko A.N., Portenko S.A., Gus’kov A.S., Pochtareva E.S., Savel’eva I.V., Volynkina A.S., Savel’ev V.N., Mikhailova M.E., Kuznetsova I.V., Bobenko O.A., Efremenko D.V., Kazakova E.S., Krasovskaya T.Yu., Kuklev V.E., Kas’yan I.A., Bil’ko E.A., Mitsevich E.V., Mitsevich I.P., Platonov M.E., Teimurazov M.G., Polosenko O.V., Eldinova V.E., Boiko E.A., Malay V.I., Klindukhov V.P., Grechanaya T.V., Nikolaevich P.N., Biryukov V.A., Bozhko I.I., Shcherbina L.I., Pogudina O.A. [Management of the investigation and detection of Legionellosis agent in the environmental samples during the preparation and hold- ing of the XXII Winter Olympics and XI Paralympics in Sochi]. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections]. 2015; 2:50–3. DOI: 10.21055/0370-1069- 2015-2-50-53. 3. Kazakova E.S., Portenko S.A., Sharova I.N., Karnaukhov I.G., Krasovskaya T.Yu., Kuklev V.E., Naidenova E.V., Bil’ko E.A., Kas’yan I.A., Shcherbakova S.A., Toporkov A.V. [Management of diagnostic investigations at the premises of specialized anti-epidemic team mo- bile complex at the time of running mass events]. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections]. 2014; 2:89–93. DOI: 10.21055/0370-1069-2014-2-89-93. 4. Kuz’kin B.P., Kulichenko A.N., Maletskaya O.V., Efremenko D.V., Manin E.A., Kotenev E.S., Ryazanova A.G., Kuznetsova I.V., Dikova S.P., Lisitskaya Ya.V., Volynkina A.S., Ponomarenko D.G., Eldinova V.E., Boiko E.A., Klindukhov V.P., Orobey V.G., Kutyrev V.V., Kazakova E.S., Kuklev V.E., Dyatlov I.A., Kartsev N.N. [Performance of the SAET of the Stavropol Anti-Plague Institute of the Rospotrebnadzor during the XXII Olympic and XI Paralympic Winter Games in Sochi]. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections]. 2015; 2:17–21. DOI: 10.21055/0370-1069-2015-2-17-21. 5. Kulichenko A.N., Volynkina A.S., Lisitskaya Ya.V., Kotenev E.S., Kuznetsova I.V., Podkolzin A.T., Zaitseva E.V., Parkina N.V., orobey V.G. [Genetic profiling of relevant for the region of cityresort Sochi agents of natural-focal and intestinal infections]. Bakteriologiya. 2016; 1(1):16–21. DOI: 10.20953/2500-1027-2016- 1-16-21.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|