The conserved biochemical activity and function of an early metazoan phosphatidylinositol 5 phosphate 4-kinase regulates growth and development

Abstract

AbstractThe ability to co-ordinate function between multiple cells is a critical requirement for multi-cellularity. This co-ordination is mediated by hormones or growth factors, molecules secreted by one cell type that can convey information to the other cells and influence their behaviour. Hormone-dependent signalling is mediated by second messenger systems;phosphoinositides (PIs) generated by lipid kinase activity are one such key second messenger system. Phosphatidylinositol 5 phosphate 4-kinase (PIP4K) is a lipid kinase that phosphorylates phosphatidylinositol 5-phosphate (PI5P) to generate phosphatidylinositol 4,5 bisphosphate [PI(4,5)P2]. Following a comprehensive bioinformatics analysis of ca. 23296 proteomes covering the tree of life, we find that PIP4K is a metazoan-specific enzyme, although its homologs are also found in choanoflagellate genomes. To understand their function in early metazoans, we experimentally analysed the biochemical activity and physiological function of PIP4K from several early metazoans. We find that the PIP4K enzyme from an early branching metazoan spongeAmphimedon queenslandica(AqPIP4K), regarded as the earliest evolved metazoan, shows a biochemical activity highly conserved with human PIP4K; AqPIP4K is able to selectively phosphorylate PI5P to generate PI(4,5)P2just as effectively as the human enzyme. Further, AqPIP4K was able to rescue the reduced cell size, growth and development phenotype in larvae of a null mutant inDrosophilaPIP4K. These phenotypes are regulated through activity of the hormone insulin, acting via the cell surface insulin receptor, a member of the receptor tyrosine kinase family, that is unique to metazoans. Together, our findings indicate that in early metazoans, AqPIP4K is likely to function in a signal transduction pathway that is required for receptor tyrosine kinase signalling. Overall, our work defines PIP4K as a signal transduction motif required to regulate receptor tyrosine kinase signalling for intercellular communication in the earliest forms of metazoa.