In-Flights of Outbreak Populations of Mountain Pine Beetle Alter the Local Genetic Structure of Established Populations a Decade After Range Expansion

Abstract

AbstractMountain pine beetles began to appear at epidemic levels in Alberta, Canada, in 2006, following six years of extensive outbreaks in neighboring British Columbia. We assessed the effect of genetic MPB in-flights from the peak of the outbreak on the genetic structure of established populations of MPB and the change over time in novel regions colonized by these inflights. We used five locations sampled during the peak of the outbreak (2005/2007) and re-sampled in 2016. We performed a ddRADseq protocol to generate a SNP dataset via single-end Illumina sequencing. We detected a northern and southern genetic cluster in both sampling sets (2005/2007 and 2016) and a demographic shift in cluster assignment after ∼10 generations from south to north in two of the sites in the path of the northern outbreak. Fst values were significantly different between most sites in the same years and between the same sites at different years, with some exceptions for northern sites established by inflights. Overall, sites in the spreading path of the MPB outbreak have taken on the genetic structure of the contiguous northern outbreak except for an isolated site in Golden, BC, and in Mount Robson Provincial Park where populations are admixed between north and south. Our results suggest that range expansion during insect outbreaks can alter the genetic structure of established populations and lead to interbreeding between populations.