Comparative Analysis of COVID-19 Serological testing methods to Polymerase Chain Reaction: A systematic review and meta-analysis

Author:

Chisompola DavidORCID,Maleti AlexORCID,Tembo KingsleyORCID,Banda Tioni,Chishinji George,Phiri RichardORCID

Abstract

AbstractBackgroundAccurate and reliable diagnosis of COVID-19 is essential for effective disease management and public health interventions. SARS-CoV-2 antibody/antigen tests play a crucial role in identifying covid-19 infections and assessing immunity in populations. This systematic review aimed to evaluate the sensitivity, specificity, and accuracy of SARS-CoV-2 antibody tests in diagnosing COVID-19.MethodA comprehensive systematic review of the literature was conducted using prominent scientific databases, including PubMed, Google Scholar, Cochrane, and Scopus to search studies published from January 2020 to May 2023. The review followed the PRISMA-DTA framework to ensure transparency and rigor in the selection and evaluation of studies. The QUADAS-2 tool was utilized to assess study quality and risk of bias. This review was registered on PROSPERO (registration number CRD 42023445695).ResultsThe pooled sensitivity of SARS-CoV-2 antibody/antigen tests was found to be 73% (95% CI: 60–86), with individual studies reporting a wide range from 30% to 100%. The pooled specificity was 98% (95% CI: 97–100), with values ranging from 85.9% to 100%. The pooled accuracy was 88%, showing variation from 50% to 100% across different studies.ConclusionThis review highlights the moderate to high sensitivity and specificity of SARS-CoV-2 antibody/antigen tests for COVID-19 diagnosis. The substantial variability in test performance necessitates the standardization of testing protocols and further research to improve accuracy and reliability. These findings offer valuable insights for clinical decision-making and the formulation of effective public health strategies related to COVID-19 diagnosis.

Publisher

Cold Spring Harbor Laboratory

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