Abstract
AbstractAnalysis of the protein composition that make upC. difficilespores is essential in understanding the protein composition of its surface layers however the spore surface is composed of protein complexes hindering protein analysis. Here we compare commonly used protein extraction methods used to extract proteins ofC. difficilespores. Our results provide a systematic analysis of how two commonly used spore coat / exosporium extraction methods compare in efficiency of protein removal and spore structural analysis.
Publisher
Cold Spring Harbor Laboratory