Palynological studies on the selected members ofFabaceae

Abstract

ABSTRACTThe investigation into pollen biology encompasses various stages such as pollen production, transfer to stigma, and the ensuing interactions leading to fertilization. Stigma plays a crucial role in providing an optimal environment for pollen germination and subsequent tube growth. However, conductingin vivostudies presents challenges due to the complexities of pistillate tissues, thus necessitating a reliance onin vitrocultures to comprehend pollen physiology. This study focuses on assessing pollen viability and germination in a selection ofFabaceaespecies, includingCanavalia gladiata, Centrosoma pubescens, Clitoria ternatea, Crotalaria pallida, Glyricidia maculata, Pueraria phaseoloides, andVigna unguiculata, utilizing tetrazolium testing and hanging drop culture methods. Results demonstrate a range of variability in pollen viability (from 19.38% to 80.78%) and germination (ranging from 23.90% to 79.81%), withCrotalaria pallidaexhibiting the highest rates of viability and germination, whereasClitoria ternateaandPueraria phaseoloidesdisplayed the lowest rates. This investigation contributes valuable insights into the pollen characteristics ofFabaceaespecies, hinting at possible connections between pollen viability/germination and seed/fruit set percentages.